Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Richter, J.P.; Goroncy, A.K.; Ronimus, R.S.; Sutherland-Smith, A.J.
    The Structural and functional characterization of mammalian ADP-dependent glucokinase (2016), J. Biol. Chem., 291, 3694-3704.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.1.147 structures reveal a ribokinase-like tertiary fold similar to archaeal orthologues but with significant differences in some secondary structural elements. Both the unliganded and the AMP-bound ADPGK structures are in the open conformation and reveal the presence of a disulfide bond between conserved cysteines that is positioned at the nucleotide-binding loop. The AMP-bound structure defines the nucleotide-binding site with one of the disulfide bond cysteines coordinating the AMP with its main chain atoms Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.147 D481A greatly reduced activity Homo sapiens
2.7.1.147 D481E greatly reduced activity Homo sapiens
2.7.1.147 D481N greatly reduced activity Homo sapiens
2.7.1.147 D84A greatly reduced activity Homo sapiens
2.7.1.147 H264A greatly reduced activity, no substrate inhibition with glucose concentrations of up to 55 mM Homo sapiens
2.7.1.147 H382A no catalytic activity Homo sapiens
2.7.1.147 H382V/H387V no catalytic activity Homo sapiens
2.7.1.147 H387A no catalytic activity Homo sapiens
2.7.1.147 R228A greatly reduced activity Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.147 ADP
-
 Homo sapiens
2.7.1.147 AMP
-
 Homo sapiens
2.7.1.147 D-glucose
-
 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.147 0.23
-
 ADP mutant R228A, pH 7.0, 37°C Homo sapiens
2.7.1.147 0.27
-
 D-glucose mutant R228A, pH 7.0, 37°C Homo sapiens
2.7.1.147 0.48
-
 D-glucose pH 7.0, 37°C Homo sapiens
2.7.1.147 0.56
-
 ADP pH 7.0, 37°C Homo sapiens
2.7.1.147 2.9
-
 GDP pH 7.0, 37°C Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.147 Homo sapiens Q9BRR6
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.147 ADP + D-glucose
-
 Homo sapiens AMP + D-glucose 6-phosphate
-
 ?
2.7.1.147 GDP + D-glucose
-
 Homo sapiens GMP + D-glucose 6-phosphate
-
 ?
2.7.1.147 additional information poor activity with CDP. No substrates: ATP, 2-deoxyglucose, L-glucose, methyl-beta-D-xylopyranoside, D-glucosamine, L-rhamnose, D-tagatose, 3-O-methyl-D-glucopyranoside, 1-O-methyl-beta-D-glucopyranoside, 1-O-methyl-alpha-D-glucopyranoside, D-arabinose, L-arabinose, myo-inositol, D-ribose, D-xylose, D-galactose, D-fructose, D-mannose, D-fructose-6-phosphate, and alpha-D-glucose-1-phosphate Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.147 ADPGK
-
 Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.147 7.5
-
-
 Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.1.147 0.5
-
 AMP pH 7.0, 37°C Homo sapiens
2.7.1.147 2.9
-
 D-glucose pH 7.0, 37°C Homo sapiens
2.7.1.147 9.1
-
 ADP pH 7.0, 37°C Homo sapiens